FOXE3 Polyclonal antibody

FOXE3 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55301-1-AP

Synonyms

ASMD, FKHL12, forkhead box E3, Forkhead box protein E3, FOXE3, FREAC 8, FREAC8

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Validation Data Gallery

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  WB analysis of HepG2 using 55301-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55301-1-AP (FOXE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 55301-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 55301-1-AP (FOXE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 55301-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 55301-1-AP (FOXE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 55301-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55301-1-AP (FOXE3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 55301-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55301-1-AP (FOXE3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HepG2 cells, A375 cells, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55301-1-AP targets FOXE3 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: forkhead box E3
• Calculated Molecular Weight: 33 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: NM_012186
• Gene Symbol: FOXE3
• Gene ID (NCBI): 2301
• RRID: AB_10896313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXE3, also named as FKHL12 and FREAC8, is a forkhead transcription factor expressed in the lens which is located at chromosome 1p32, have been associated with both recessive and dominant ocular disease. Defects in FOXE3 are a cause of anterior segment mesenchymal dysgenesis (ASMD). Defects in FOXE3 are a cause of congenital primary aphakia (CPA). This antibody is specific to FOXE3.