FOXE1 Polyclonal antibody

FOXE1 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 55363-1-AP

Synonyms

FKHL15, Forkhead box protein E1, Forkhead box protein E2, FOXE1, FOXE2, HFKH4, HFKL5, HNF 3/fork head like protein 5, Thyroid transcription factor 2, TITF2, TTF 2, TTF2

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Validation Data Gallery

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  IHC staining of human thyroid cancer using 55363-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55363-1-AP (FOXE1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 55363-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55363-1-AP (FOXE1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive IHC detected in: human thyroid cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

55363-1-AP targets FOXE1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: forkhead box E1 (thyroid transcription factor 2)
• Calculated Molecular Weight: 38 kDa
• GenBank Accession Number: NM_004473
• Gene Symbol: FOXE1
• Gene ID (NCBI): 2304
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXE1 is a thyroid-specific forkhead transcription factor essential for thyroid gland development, as well as for the maintenance of the thyroid differentiated state in adults. Mutations in FOXE1 are associated with congenital hypothyroidism and cleft palate with thyroid dysgenesis (PMID: 20453517, PMID: 25381600). FOXE1 is expressed in the developing thyroid and the anterior pituitary gland.

Publications

Species	Application	Title
human	WB	Cancer Lett; Epigenetic inhibition of CTCF by HN1 promotes dedifferentiation and stemness of anaplastic thyroid cancer; Authors - Zongfu Pan