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  • Featured Product
  • KD/KO Validated

FOXD3 Polyclonal antibody, PBS Only

FOXD3 Polyclonal Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

Cat no : 27878-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using FOXD3 Polyclonal antibody (27878-1-AP)

    WB analysis of HEK-293 using 27878-1-AP (same clone as 27878-1-PBS)

    WB result of FOXD3 antibody (27878-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FOXD3 transfected HEK-293 cells. This data was developed using the same antibody clone with 27878-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FOXD3 Polyclonal antibody (27878-1-AP)

    WB analysis of HEK-293 using 27878-1-AP (same clone as 27878-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27878-1-AP (FOXD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27878-1-PBS in a different storage buffer formulation.

Product Information

27878-1-PBS targets FOXD3 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXD3 fusion protein Ag27260
Full Name forkhead box D3
Calculated Molecular Weight 48 kDa
Observed Molecular Weight57-60 kDa
GenBank Accession NumberNM_012183.2
Gene Symbol FOXD3
Gene ID (NCBI) 27022
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

FOXD3, also named as HFH2, belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. FOXD3 binds to the consensus sequence 5'-A[AT]T[AG]TTTGTTT-3' and acts as a transcriptional repressor. It also acts as a transcriptional activator. FOXD3 promotes development of neural crest cells from neural tube progenitors. It restricts neural progenitor cells to the neural crest lineage while suppressing interneuron differentiation. It required for maintenance of pluripotent cells in the pre-implantation and peri-implantation stages of embryogenesis. FOXD3 has one isoform produced by alternative splicing with the MW of 47 kDa. It can be detected as 57-60 kDa in some literature(PMID: 31940493).