FOXC2 Recombinant antibody, PBS Only

FOXC2 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240403A2

Cat no : 83476-6-PBS

Synonyms

MFH 1, Mesenchyme fork head protein 1, Forkhead-related protein FKHL14, Forkhead box protein C2, FKHL14

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Validation Data Gallery

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  WB analysis using 83476-6-RR (same clone as 83476-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83476-6-RR (FOXC2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83476-6-PBS in a different storage buffer formulation.

• 

  FC experiment of HeLa using 83476-6-RR (same clone as 83476-6-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug FOXC2 Recombinant antibody (83476-6-RR, Clone:240403A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83476-6-PBS in a different storage buffer formulation.

• 

  FC experiment of A549 using 83476-6-RR (same clone as 83476-6-PBS)

  1x10^6 A549 cells were intracellularly stained with 0.25 ug FOXC2 Recombinant antibody (83476-6-RR, Clone:240403A2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83476-6-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83476-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83476-6-RR against Human FOXC2 were performed. The affinity constant is 0.328 nM.

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Product Information

The immunogen of 83476-6-PBS is FOXC2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FOXC2 fusion protein Ag33121
• Full Name: forkhead box C2 (MFH-1, mesenchyme forkhead 1)
• Calculated Molecular Weight: 54kd
• Observed Molecular Weight: 54-68 kDa
• GenBank Accession Number: NM_005251
• Gene Symbol: FOXC2
• Gene ID (NCBI): 2303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Forkhead box protein C2 (FOXC2) also known as forkhead-related protein FKHL14 (FKHL14), transcription factor FKH-14, or mesenchyme fork head protein 1 (MFH1) is a protein that in humans is encoded by the FOXC2 gene. FOXC2 is a member of the fork head box (FOX) family of transcription factors. FOX transcription factors are expressed during development and are associated with a number of cellular and developmental differentiation processes. FOXC2 is required during early development of the kidneys, including differentiation of podocytes and maturation of the glomerular basement membrane. It is also involved in the early development of the heart. FOXC2 is also involved in cancer metastases. In particular, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells. (PMID: 8674414 9169153 19935708) This antibody recognizes the 56 kDa FOXC2 protein, but the phosphorylated FOXC2 may be a 56-65 kDa protein. The 62kDa band was also detected in the study(PMID: 19540201).