FOXC2 Monoclonal antibody, PBS Only

FOXC2 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3E3E1

Cat no : 68384-1-PBS

Synonyms

FKHL14, Forkhead box protein C2, FOXC2, LD, Mesenchyme fork head protein 1, MFH 1, MFH 1 protein, MFH1, Transcription factor FKH 14

---

Validation Data Gallery

• 

  WB analysis of hTERT-RPE1 using 68384-1-Ig (same clone as 68384-1-PBS)

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68384-1-Ig (FOXC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68384-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68384-1-Ig (same clone as 68384-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68384-1-Ig (FOXC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 68384-1-Ig (same clone as 68384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68384-1-Ig (FOXC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68384-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 68384-1-Ig (same clone as 68384-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68384-1-Ig (FOXC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68384-1-PBS in a different storage buffer formulation.

---

Product Information

68384-1-PBS targets FOXC2 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, Pig samples.

• Tested Reactivity: Human, Rat, Pig
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FOXC2 fusion protein Ag19378
• Full Name: forkhead box C2 (MFH-1, mesenchyme forkhead 1)
• Calculated Molecular Weight: 501 aa, 54 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC113437
• Gene Symbol: FOXC2
• Gene ID (NCBI): 2303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

Forkhead box protein C2 (FOXC2) also known as forkhead-related protein FKHL14 (FKHL14), transcription factor FKH-14, or mesenchyme fork head protein 1 (MFH1) is a protein that in humans is encoded by the FOXC2 gene. FOXC2 is a member of the fork head box (FOX) family of transcription factors. FOX transcription factors are expressed during development and are associated with a number of cellular and developmental differentiation processes. FOXC2 is required during early development of the kidneys, including differentiation of podocytes and maturation of the glomerular basement membrane. It is also involved in the early development of the heart. FOXC2 is also involved in cancer metastases. In particular, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells. (PMID: 8674414 9169153 19935708)