FOXC2 Polyclonal antibody

• Featured Product
• KD/KO Validated

FOXC2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 23066-1-AP

Synonyms

MFH1, MFH 1, Mesenchyme fork head protein 1, Forkhead-related protein FKHL14, Forkhead box protein C2

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Validation Data Gallery

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  WB analysis using 23066-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23066-1-AP (FOXC2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 23066-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 23066-1-AP (FOXC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of A375 using 23066-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using 23066-1-AP (FOXC2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HCT 116 cells, rat spleen tissue, MCF-7 cells
• Positive IF/ICC detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Product Information

23066-1-AP targets FOXC2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOXC2 fusion protein Ag19378
• Full Name: forkhead box C2 (MFH-1, mesenchyme forkhead 1)
• Calculated Molecular Weight: 501 aa, 54 kDa
• Observed Molecular Weight: 54-68 kDa
• GenBank Accession Number: BC113437
• Gene Symbol: FOXC2
• Gene ID (NCBI): 2303
• RRID: AB_2879204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Forkhead box protein C2 (FOXC2) also known as forkhead-related protein FKHL14 (FKHL14), transcription factor FKH-14, or mesenchyme fork head protein 1 (MFH1) is a protein that in humans is encoded by the FOXC2 gene. FOXC2 is a member of the fork head box (FOX) family of transcription factors. FOX transcription factors are expressed during development and are associated with a number of cellular and developmental differentiation processes. FOXC2 is required during early development of the kidneys, including differentiation of podocytes and maturation of the glomerular basement membrane. It is also involved in the early development of the heart. FOXC2 is also involved in cancer metastases. In particular, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells. (PMID: 8674414 9169153 19935708) This antibody recognizes the 56 kDa FOXC2 protein, but the phosphorylated FOXC2 may be a 56-65 kDa protein. The 62kDa band was also detected in the study(PMID: 19540201).

Publications

Species	Application	Title
human	WB	Theranostics; Hypoxia-induced feedback of HIF-1α and lncRNA-CF129 contributes to pancreatic cancer progression through stabilization of p53 protein.; Authors - Mingliang Liu ; KD Validated
mouse	WB	Clin Transl Med; VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.; Authors - Qiu-Yue Lin
human	WB,IF	Exp Cell Res; Long non-coding RNA GClnc1 knockdown suppresses progression of epithelial ovarian cancer by recruiting FOXC2 to disrupt the NOTCH1/NF-κB/Snail pathway.; Authors - Dandan Wu
rat	WB	Am J Transl Res; Naringin promotes osteogenic differentiation of bone marrow stromal cells by up-regulating Foxc2 expression via the IHH signaling pathway.; Authors - Fei-Xiang Lin
	WB	Acta Biochim Biophys Sin (Shanghai); STK33 plays an important positive role in the development of human large cell lung cancers with variable metastatic potential.; Authors - Ping Wang
human	WB,IHC	Cell Death Dis; Decreased ZNF750 promotes angiogenesis in a paracrine manner via activating DANCR/miR-4707-3p/FOXC2 axis in esophageal squamous cell carcinoma.; Authors - Yanghui Bi