FOXA1 Recombinant antibody, PBS Only (Capture/Detector)

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  Sandwich ELISA standard curve of MP01842-3

  Sandwich ELISA standard curve of MP01842-3, Human FOXA1 Recombinant Matched Antibody Pair - PBS only. 85125-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag14243. 85125-1-PBS was HRP conjugated as the detection antibody. Range: 0.195-12.5 ng/mL.

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  Sample test of MP01842-3

  The mean FOXA1 concentration was determined to be 32.58 ng/mL in HepG2 cell extract based on a 1.00 mg/mL extract load, 90.93 ng/mL in MDA-MB-468 cell extract based on a 3.20 mg/mL extract load and 341.89 ng/mL in MCF-7 cell extract based on a 3.50 mg/mL extract load.

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  Cytometric bead array standard curve of MP01842-2

  Cytometric bead array standard curve of MP01842-2, FOXA1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85125-1-PBS. Detection antibody: 85125-3-PBS. Standard: Ag14243. Range: 0.156-20 ng/mL.

FOXA1 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 85125-1-PBS

Clone No.242658A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

HNF 3A, HNF 3 alpha, Hepatocyte nuclear factor 3-alpha, Forkhead box protein A1, forkhead box A1

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Product Information

85125-1-PBS targets FOXA1 as part of a matched antibody pair:

MP01842-2: 85125-1-PBS capture and 85125-3-PBS detection (validated in Cytometric bead array)

MP01842-3: 85125-4-PBS capture and 85125-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FOXA1 fusion protein Ag14243
• Full Name: forkhead box A1
• Calculated Molecular Weight: 473 aa, 49 kDa
• GenBank Accession Number: BC033890
• Gene Symbol: FOXA1
• Gene ID (NCBI): 3169
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P55317
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.