FNIP2 Polyclonal antibody

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  IP experiment of HEK-293 using 31283-1-AP

  IP result of anti-FNIP2 (IP:31283-1-AP, 4ug; Detection:31283-1-AP 1:500) with HEK-293 cells lysate 1470 ug.

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  IHC staining of mouse kidney using 31283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31283-1-AP (FNIP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 31283-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31283-1-AP (FNIP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FNIP2 Polyclonal Antibody for IP, IHC, ELISA

Cat No. 31283-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

IP, IHC, ELISA

Synonyms

FNIP2, FNIPL, KIAA1450, FNIP1 like protein

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Tested Applications

• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31283-1-AP targets FNIP2 in IP, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FNIP2 fusion protein Ag35014
• Full Name: folliculin interacting protein 2
• Observed Molecular Weight: 125 kDa
• GenBank Accession Number: BC166693
• Gene Symbol: FNIP2
• Gene ID (NCBI): 57600
• RRID: AB_3669929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9P278
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Folliculin (FLCN)-interacting proteins 1 and 2 (FNIP1, FNIP2) are homologous binding partners of FLCN, a tumor suppressor for kidney cancer. FNIP2 was found to bind to the C terminus of FLCN and to AMPK, like FNIP1. FNIP2 competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (PMID: 18403135).