FNIP1 Polyclonal antibody

FNIP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28380-1-AP

Synonyms

DKFZp686E18167, DKFZp781P0215, FNIP1, KIAA1961

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Validation Data Gallery

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  WB analysis using 28380-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28380-1-AP (FNIP1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. .

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  IF Staining of C2C12 using 28380-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using FNIP1 antibody (28380-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IF/ICC detected in: C2C12 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28380-1-AP targets FNIP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FNIP1 fusion protein Ag28976
• Full Name: folliculin interacting protein 1
• Calculated Molecular Weight: 508 aa, 57 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: BC001956
• Gene Symbol: FNIP1
• Gene ID (NCBI): 96459
• RRID: AB_2881128
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Folliculin-interacting protein 1 (FNIP1) forms a complex with the protein folliculin (FLCN), together acting as guanosine triphosphate (GTP)-activating protein (GAP) for RagC. The FNIP1-FLCN complex has emerged as an amino acid sensor to the mechanistic target of rapamycin complex 1 (mTORC1), involved in how amino acids control TFEB activation. AMPK phosphorylation of FNIP1 induces lysosomal and mitochondrial biogenesis (PMID: 37079666)

Publications

Species	Application	Title
mouse	WB	Bioact Mater; Glucose-responsive, antioxidative HA-PBA-FA/EN106 hydrogel enhanced diabetic wound healing through modulation of FEM1b-FNIP1 axis and promoting angiogenesis; Authors - Wenqian Zhang