FMO5 Polyclonal antibody

• Featured Product
• KD/KO Validated

FMO5 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 13699-1-AP

Synonyms

Dimethylaniline oxidase 5, FMO 5, FMO5

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Validation Data Gallery

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  WB analysis of human liver using 13699-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13699-1-AP (FMO5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 13699-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13699-1-AP (FMO5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse lung using 13699-1-AP

  IP result of anti-FMO5 (IP:13699-1-AP, 4ug; Detection:13699-1-AP 1:500) with mouse lung tissue lysate 4000ug.

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  IHC staining of human breast cancer using 13699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13699-1-AP (FMO5 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 13699-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13699-1-AP (FMO5 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human liver tissue, mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below

Product Information

13699-1-AP targets FMO5 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FMO5 fusion protein Ag4604
• Full Name: flavin containing monooxygenase 5
• Calculated Molecular Weight: 533 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC035687
• Gene Symbol: FMO5
• Gene ID (NCBI): 2330
• RRID: AB_2105409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Microsomal flavin-containing monooxygenases (FMOs) [dimethylaniline monooxygenase (N-oxide forming) catalyze the FAD-, NADPH- and O2-dependent oxidation of a large number of structurally diverse compounds, including drugs, pesticides, and industrial chemicals containing a soft nucleophile(PMID:12488558 ). FMO5, which belongs to the FMO family, is a lesser component of human liver microsomes and is present at about one-third the level of FMO3. FMO5 protein is also present at very low levels in kidney, however, FMO5 exhibits a severely restricted substrate specificity for most drugs and other xenobiotics examined to date(PMID:10950857). It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Flavin-Containing Monooxygenases Are Conserved Regulators of Stress Resistance and Metabolism.; Authors - Shijiao Huang
mouse	WB	Biochem Pharmacol; The phenotype of a knockout mouse identifies flavin-containing monooxygenase 5 (FMO5) as a regulator of metabolic ageing.; Authors - Sandra G Gonzalez Malagon ; KO Validated
mouse	WB	Drug Metab Dispos; The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.; Authors - Min Chen
mouse	WB	Front Pharmacol; Puerarin ameliorates non-alcoholic fatty liver disease by inhibiting lipid metabolism through FMO5; Authors - Zhaoyi Li