FMO2 Monoclonal antibody, PBS Only

FMO2 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

3F1F7

Cat no : 67019-1-PBS

Synonyms

Dimethylaniline oxidase 2, FLJ40826, FMO 1B1, FMO 2, FMO1B1, FMO2

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Validation Data Gallery

• 

  WB analysis of mouse lung using 67019-1-Ig (same clone as 67019-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 67019-1-Ig (FMO2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67019-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 67019-1-Ig (same clone as 67019-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 67019-1-Ig (FMO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67019-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig lung using 67019-1-Ig (same clone as 67019-1-PBS)

  pig lung tissue were subjected to SDS PAGE followed by western blot with 67019-1-Ig (FMO2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67019-1-Ig (same clone as 67019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67019-1-Ig (FMO2 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67019-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 67019-1-Ig (same clone as 67019-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67019-1-Ig (FMO2 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67019-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A549 using 67019-1-Ig (same clone as 67019-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using FMO2 antibody (67019-1-Ig, Clone: 3F1F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67019-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67019-1-PBS targets FMO2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Pig samples.

• Tested Reactivity: Human, Mouse, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FMO2 fusion protein Ag8610
• Full Name: flavin containing monooxygenase 2
• Calculated Molecular Weight: 471aa,54 kDa; 534aa,61 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC005894
• Gene Symbol: FMO2
• Gene ID (NCBI): 2327
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.