FMO2 Polyclonal antibody

FMO2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15725-1-AP

Synonyms

Dimethylaniline oxidase 2, FMO 1B1, FMO 2, FMO1B1, FMO2

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Validation Data Gallery

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  WB analysis using 15725-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15725-1-AP (FMO2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 15725-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15725-1-AP (FMO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15725-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15725-1-AP (FMO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15725-1-AP targets FMO2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FMO2 fusion protein Ag8464
• Full Name: flavin containing monooxygenase 2
• Calculated Molecular Weight: 471aa,54 kDa; 534aa,61 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC005894
• Gene Symbol: FMO2
• Gene ID (NCBI): 2327
• RRID: AB_2278518
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FMO2, belongs to FMO (flavin containing monooxygenase) family, which catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. FMO family has 1-5 members, with homologous sequences. This antibody is specially against FMO2. It does not bind other FMOs.