FMNL3 Polyclonal antibody, PBS Only

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  WB analysis using 29756-1-AP (same clone as 29756-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 29756-1-AP (FMNL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29756-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29756-1-AP (same clone as 29756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29756-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 29756-1-AP (same clone as 29756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29756-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 29756-1-AP (same clone as 29756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29756-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse liver using 29756-1-AP (same clone as 29756-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29756-1-PBS in a different storage buffer formulation.

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  IF Staining of HUVEC using 29756-1-AP (same clone as 29756-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FMNL3 antibody (29756-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 29756-1-PBS in a different storage buffer formulation.

FMNL3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 29756-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

29756-1-PBS targets FMNL3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FMNL3 fusion protein Ag30543
• Full Name: formin-like 3
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: NM_175736
• Gene Symbol: FMNL3
• Gene ID (NCBI): 91010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IVF7
• Storage Buffer: PBS only , pH 7.3
• Storage Conditions: Store at -80°C.

Background Information

FMNL3 plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the control of cell shape and migration. In quiescent endothelial cells, FMNL3 triggers rearrangement of the actin cytoskeleton, but does not alter microtubule alignement.