FMNL3 Polyclonal antibody

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  WB analysis using 29756-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29756-1-AP (FMNL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 29756-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 29756-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 29756-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 29756-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29756-1-AP (FMNL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HUVEC using 29756-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using FMNL3 antibody (29756-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

FMNL3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 29756-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DKFZp762B245, FHOD3, FLJ45265, FMNL3, formin like 3, Formin like protein 3, KIAA2014, WBP 3, WBP3, WW domain binding protein 3

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Tested Applications

• Positive WB detected in: mouse liver tissue, rat liver tissue
• Positive IHC detected in: human colon cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29756-1-AP targets FMNL3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FMNL3 fusion protein Ag30543
• Full Name: formin-like 3
• Calculated Molecular Weight: 117 kDa
• Observed Molecular Weight: 117 kDa
• GenBank Accession Number: NM_175736
• Gene Symbol: FMNL3
• Gene ID (NCBI): 91010
• RRID: AB_3086155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IVF7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FMNL3 plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the control of cell shape and migration. In quiescent endothelial cells, FMNL3 triggers rearrangement of the actin cytoskeleton, but does not alter microtubule alignement.