FMNL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FMNL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 27834-1-AP

Synonyms

C17orf1, C17orf1B, CLL associated antigen KW 13, FHOD4, FMNL, FMNL1, formin like 1, Formin like protein 1, KW 13, Leukocyte formin

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Validation Data Gallery

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  WB analysis using 27834-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27834-1-AP (FMNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 27834-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27834-1-AP (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of Caki-1 using 27834-1-AP

  IP result of anti-FMNL1 (IP:27834-1-AP, 2ug; Detection:27834-1-AP 1:2000) with Caki-1 cells lysate 1000 ug. The result was provided by Keli Chai, Xuzhou Medical University.

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  IHC staining of human lymphoma using 27834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27834-1-AP (FMNL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 27834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27834-1-AP (FMNL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 27834-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 27834-1-AP (FMNL1 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: Raji cells, Jurkat cells, HeLa cells
• Positive IP detected in: Caki-1
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

27834-1-AP targets FMNL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FMNL1 fusion protein Ag27312
• Full Name: formin-like 1
• Observed Molecular Weight: 125-150 kDa
• GenBank Accession Number: BC111564
• Gene Symbol: FMNL1
• Gene ID (NCBI): 752
• RRID: AB_2880987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 27834-1-AP is a rabbit polyclonal antibody recognises the 125-150 kDa protein.(PMID:16494944)

Publications

Species	Application	Title
human	WB	Theranostics; Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer; Authors - Chen Li ; KD Validated
human	IHC	Gastric Cancer; FHOD1 and FMNL1 formin proteins in intestinal gastric cancer: correlation with tumor-infiltrating T lymphocytes and molecular subtypes.; Authors - Naziha Mansuri
human	WB	Cell Rep; Substrate rigidity dictates colorectal tumorigenic cell stemness and metastasis via CRAD-dependent mechanotransduction.; Authors - Yuhan Chang
human	IHC	Biomed Res Int; Systematic Characterization of Expression Patterns and Immunocorrelations of Formin-Like Genes in Breast Cancer; Authors - Erli Gao
mouse	IHC	Clin Immunol; Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy; Authors - Jie Mei
human	IF	J Cancer; Formin protein FMNL1 is a biomarker for tumor-infiltrating immune cells and associated with well immunotherapeutic response; Authors - Guomin Lu