NeutraKine FLT3 Ligand Monoclonal antibody

NeutraKine FLT3 Ligand Monoclonal Antibody for ELISA, Neutralization

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

ELISA, Neutralization

Conjugate

Unconjugated

CloneNo.

3E1A3

Cat no : 69032-1-Ig

Synonyms

FL, Flt3 ligand, Flt3L, FLT3LG, SL cytokine

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Validation Data Gallery

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  Neutralization experiment of NeutraKine FLT3 Ligand using 69032-1-Ig

  Recombinant human FLT3 Ligand (Cat.NO. HZ-1151) stimulates proliferation of OCI-AML5 cells in a dose-dependent manner (blue curve, refer to bottom X-left Y). The activity of human FLT3 (1 ng/mL) is neutralized by mouse anti-human FLT3 Ligand monoclonal antibody 69032-1-Ig at serial dose (red curve, refer to top X-right Y). The ND50 is typically 0.01-0.05 μg/mL.The control mouse anti-human FLT3 Ligand monoclonal antibody 69532-1-Ig could recognize human FLT3 Ligand, but could not neutralize human FLT3 Ligand at serial does at the range of 0.12207-2000ng/mL(green curve, refer to top X-right Y).

• 

  Neutralization experiment of NeutraKine FLT3 Ligand using 69032-1-Ig

  Recombinant human FLT3 Ligand (Cat.NO. HZ-1151) stimulates proliferation of OCI-AML5 cells in a dose dependent manner (blue curve, refer to bottom X-left Y axis). The activity of human FLT3 Ligand (1ng/mL HZ-1151) is neutralized by mouse anti-human FLT3 Ligand monoclonal antibody 69032-1-Ig at serial dose (red curve, refer to top X-right Y axis). The ND50 is typically 0.01-0.05 μg/mL.

• 

  ELISA experiment of Recombinant protein using 69032-1-Ig

  Indirect ELISA was carried out by coating recombinant Human FLT3 Ligand (Cat.NO. HZ-1151) at 70 ng/well followed by blocking and adding serial diluted FLT3 Ligand antibody 69032-1-Ig and 69532-1-Ig respectively. Signal was developed with TMB and stopped by H2SO4. Signal strength was measured by absorbance at 450 nm.

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• Neutralization: OCI-AML5

Product Information

69032-1-Ig targets NeutraKine FLT3 Ligand in ELISA, Neutralization applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: human NeutraKine FLT3 Ligand protein HZ-1151
• Full Name: fms-related tyrosine kinase 3 ligand
• Gene Symbol: FLT3 Ligand
• Gene ID (NCBI): 2323
• Conjugate: Unconjugated
• Form: Lyophilized Powder
• Purification Method: Protein G purification
• Storage Buffer: Sterile PBS.
• Endotoxin: <0.1 EU/μg
• Reconstitution: This product was lyophilized from a 0.2 μm filtered solution in PBS. Reconstitute at 1.0 mg/mL in sterile H2O before use.
• Stability and Storage: Lyophilized antibodies are stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Upon reconstitution we recommend that the solution can be stored at (4°C) for short term or at (-20°C) to (-80°C) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.

Background Information

The FMS-related tyrosine kinase 3 (FLT3) ligand (FLT3LG), a growth factor, is mainly derived from lymphocytes in the tumor microenvironment. It binds to FLT3 on DCs to enhance their differentiation and expansion. Therefore, it may assist DCs in tumor antigen presentation and antitumor immune response. In addition, mature DCs express FLT3 and proliferate massively under the trigger of FLT3LG.

This antibody is used to neutralize the bioactivity of FLT3 Ligand.