FLNA Monoclonal antibody, PBS Only

FLNA Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G4H3

Cat no : 67133-1-PBS

Synonyms

ABP 280, ABPX, Actin binding protein 280, Alpha filamin, DKFZp434P031, Filamin 1, Filamin A, FLN, FLN A, FLN1, FLNA, FMD, MNS, NHBP, Non muscle filamin, OPD, OPD1, OPD2

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Validation Data Gallery

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  WB analysis using 67133-1-Ig (same clone as 67133-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  WB analysis using 67133-1-Ig (same clone as 67133-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 67133-1-Ig (same clone as 67133-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 67133-1-Ig (same clone as 67133-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67133-1-Ig (same clone as 67133-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67133-1-Ig (FLNA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IHC staining of human cervical cancer using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67133-1-Ig (FLNA antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using FLNA antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 67133-1-Ig (same clone as 67133-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FLNA antibody (67133-1-Ig, Clone: 1G4H3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67133-1-PBS in a different storage buffer formulation.

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Product Information

67133-1-PBS targets FLNA in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: FLNA fusion protein Ag23291
• Full Name: filamin A, alpha (actin binding protein 280)
• Calculated Molecular Weight: 2647 aa, 280 kDa
• Observed Molecular Weight: 280 kDa
• GenBank Accession Number: BC014654
• Gene Symbol: FLNA
• Gene ID (NCBI): 2316
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

FLNA encodes a 280 kDa widely expressed actin binding protein called filamin A which can crosslinks actin filaments and links actin filaments to membrane glycoproteins to form a three-dimensional network (PMID:29931263). And filamin A could interact with many other proteins, involved in receptor activation, cell migration and adhesion, cell proliferation, inflammation and tumorigenesis, studies have been reported that FLNA overexpressed in multiple types of tumors, suggesting FLNA may involved in cancer aggressiveness(PMID:29100390). What's more, FLNA is an causative gene of periventricular nodular heterotopia (PNH) (PMID: 29062687)