FKBP52 Polyclonal antibody

FKBP52 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22623-1-AP

Synonyms

FKBP4, FKBP-4, FK506-binding protein 4, EC:5.2.1.8, 52 kDa FK506-binding protein

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Validation Data Gallery

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  WB analysis of PC-3 using 22623-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 22623-1-AP (FKBP52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 22623-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22623-1-AP (FKBP52 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 22623-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 22623-1-AP (FKBP52 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 22623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22623-1-AP (FKBP52 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 22623-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 22623-1-AP (FKBP52 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of PC-3 using 22623-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 22623-1-AP (FKBP52 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: PC-3 cells
• Positive IHC detected in: human colon tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

22623-1-AP targets FKBP52 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FKBP52 fusion protein Ag18704
• Full Name: FK506 binding protein 4, 59kDa
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC007924
• Gene Symbol: FKBP52
• Gene ID (NCBI): 2288
• RRID: AB_2879136
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The large molecular-weight immunophilin, FKBP52, is a known target of the immunosuppressive drug FK506. FKBP52 exhibits peptidyl-prolyl cis-trans isomerase (PPIase) activity, which is inhibited by the binding of FK506-properties that it shares with the smaller but better-studied immunophilin, FKBP12(PMID:15381148). It is essential to AR-mediated induction of genes involved in sexually dimorphic development of the penis (NKX3-1), in erectile function of the mature organ (NOS2 and NOS3), and in promotion of apoptosis during development(PMID:20605780).