FKBP5 Monoclonal antibody, PBS Only

FKBP5 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, rat, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3B11F3

Cat no : 67874-1-PBS

Synonyms

51 kDa FK506 binding protein, 51 kDa FKBP, AIG6, Androgen regulated protein 6, FF1 antigen, FK506 binding protein 5, FKBP 5, FKBP 51, FKBP5, FKBP51, FKBP54, HSP90 binding immunophilin, P54, PPIase, PPIase FKBP5, Ptg 10, Rotamase

---

Validation Data Gallery

• 

  WB analysis using 67874-1-Ig (same clone as 67874-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat testis using 67874-1-Ig (same clone as 67874-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 67874-1-Ig (same clone as 67874-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67874-1-Ig (FKBP5 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67874-1-Ig (same clone as 67874-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 67874-1-Ig (same clone as 67874-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67874-1-Ig (FKBP5 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67874-1-PBS in a different storage buffer formulation.

---

Product Information

67874-1-PBS targets FKBP5 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FKBP5 fusion protein Ag5514
• Full Name: FK506 binding protein 5
• Calculated Molecular Weight: 457 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC042605
• Gene Symbol: FKBP5
• Gene ID (NCBI): 2289
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FKBP5(Peptidyl-prolyl cis-trans isomerase FKBP5 ) interacts with functionally mature heterooligomeric progesterone receptor complexes along with HSP90 and TEBP. Unlike the predominant T-cell expression of the mouse protein, FKBP5 is expressed as a 51-kD protein in a variety of tissues but not in brain, colon, or lung(PMID: 9125197).