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FKBP5 Polyclonal antibody

FKBP5 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 14155-1-AP

Synonyms

EC:5.2.1.8, Androgen-regulated protein 6, AIG6, 54 kDa progesterone receptor-associated immunophilin, 51 kDa FK506-binding protein



Tested Applications

Positive WB detected inK-562 cells, HEK-293 cells, mouse thymus tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14155-1-AP targets FKBP5 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBP5 fusion protein Ag5337
Full Name FK506 binding protein 5
Calculated Molecular Weight 457 aa, 51 kDa
Observed Molecular Weight 51 kDa
GenBank Accession NumberBC042605
Gene Symbol FKBP5
Gene ID (NCBI) 2289
RRIDAB_2231625
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP5, also known as FKBP51, belongs to the family of immunophilins, FK506 binding proteins (FKBPs). FKBP5 is a negative regulator of GR activity and plays a key role in the termination of the stress response by GRs. FKBP5 limits GR function by decreasing ligand-binding sensitivity, thereby delaying nuclear translocation and reducing GR-dependent transcriptional activity. The known functions of FKBP5 include the regulation of steroid hormone receptors, inhibiting apoptosis in cancer cells, promoting Akt1 dephosphorylation, and inhibiting the Akt1 pathways, to name a few. FKBP5 is widely expressed and the calculated molecular weight is 51 kDa (PMID: 21119664, PMID: 34077736, PMID: 37154033).

Protocols

Product Specific Protocols
WB protocol for FKBP5 antibody 14155-1-APDownload protocol
IHC protocol for FKBP5 antibody 14155-1-APDownload protocol
IP protocol for FKBP5 antibody 14155-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Decoding the temporal and regional specification of microglia in the developing human brain.

Authors - Yanxin Li
humanWB

EMBO J

USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.

Authors - Kuntian Luo
  • KD Validated
mouseIHC

Nutrients

Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus

Authors - Tova M Bergsten
mouseIHC

Mol Cancer Ther

Cell intrinsic role of COX-2 in pancreatic cancer development.

Authors - Hill Reginald R
mouseWB

RNA

The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly.

Authors - Martinez Natalia J NJ
mouseWB

Biochem Pharmacol

Developmental nicotine exposure elicits multigenerational disequilibria in proBDNF proteolysis and glucocorticoid signaling in the frontal cortices, striata, and hippocampi of adolescent mice.

Authors - Jordan M Buck