FKBP5 Polyclonal antibody

• Featured Product
• KD/KO Validated

FKBP5 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 14155-1-AP

Synonyms

EC:5.2.1.8, Androgen-regulated protein 6, AIG6, 54 kDa progesterone receptor-associated immunophilin, 51 kDa FK506-binding protein

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Validation Data Gallery

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  WB analysis of K-562 using 14155-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 14155-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14155-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14155-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14155-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14155-1-AP (FKBP5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 14155-1-AP

  IP result of anti-FKBP5 (IP:14155-1-AP, 3ug; Detection:14155-1-AP 1:1000) with K-562 cells lysate 1500ug.

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  IHC staining of human colon cancer using 14155-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14155-1-AP (FKBP5 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 14155-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 14155-1-AP (FKBP5 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: K-562 cells, HEK-293 cells, mouse thymus tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 6 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Product Information

14155-1-AP targets FKBP5 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FKBP5 fusion protein Ag5337
• Full Name: FK506 binding protein 5
• Calculated Molecular Weight: 457 aa, 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: BC042605
• Gene Symbol: FKBP5
• Gene ID (NCBI): 2289
• RRID: AB_2231625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FKBP5, also known as FKBP51, belongs to the family of immunophilins, FK506 binding proteins (FKBPs). FKBP5 is a negative regulator of GR activity and plays a key role in the termination of the stress response by GRs. FKBP5 limits GR function by decreasing ligand-binding sensitivity, thereby delaying nuclear translocation and reducing GR-dependent transcriptional activity. The known functions of FKBP5 include the regulation of steroid hormone receptors, inhibiting apoptosis in cancer cells, promoting Akt1 dephosphorylation, and inhibiting the Akt1 pathways, to name a few. FKBP5 is widely expressed and the calculated molecular weight is 51 kDa (PMID: 21119664, PMID: 34077736, PMID: 37154033).

Publications

Species	Application	Title
human	IF	Cell Stem Cell; Decoding the temporal and regional specification of microglia in the developing human brain.; Authors - Yanxin Li
human	WB	EMBO J; USP49 negatively regulates tumorigenesis and chemoresistance through FKBP51-AKT signaling.; Authors - Kuntian Luo ; KD Validated
mouse	IHC	Nutrients; Kaempferol, a Phytoprogestin, Induces a Subset of Progesterone-Regulated Genes in the Uterus; Authors - Tova M Bergsten
mouse	IHC	Mol Cancer Ther; Cell intrinsic role of COX-2 in pancreatic cancer development.; Authors - Hill Reginald R
mouse	WB	RNA; The co-chaperones Fkbp4/5 control Argonaute2 expression and facilitate RISC assembly.; Authors - Martinez Natalia J NJ
mouse	WB	Biochem Pharmacol; Developmental nicotine exposure elicits multigenerational disequilibria in proBDNF proteolysis and glucocorticoid signaling in the frontal cortices, striata, and hippocampi of adolescent mice.; Authors - Jordan M Buck