FIZ1 Polyclonal antibody

• 

  WB analysis of Jurkat using 23257-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23257-1-AP (FIZ1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 23257-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 23257-1-AP (FIZ1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23257-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23257-1-AP (FIZ1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

FIZ1 Polyclonal Antibody for WB, ELISA

Cat No. 23257-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

FIZ1

---

Tested Applications

• Positive WB detected in: Jurkat cells, NIH/3T3 cells, HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23257-1-AP targets FIZ1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FIZ1 fusion protein Ag19760
• Full Name: FLT3-interacting zinc finger 1
• Calculated Molecular Weight: 496 aa, 52 kDa
• Observed Molecular Weight: 52 kDa
• GenBank Accession Number: BC136512
• Gene Symbol: FIZ1
• Gene ID (NCBI): 84922
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96SL8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FLT3 interacting zinc finger 1 (FIZ1) is a ubiquitous expressed protein that composes of 11 C2H2 zinc finger domains spread over the entire protein in clusters of two, three, or four fingers. The interaction of FIZ1 was mapped to the catalytic domain of FLT3, which is a receptor tyrosine kinase involved in proliferation and survival of hematopoietic progenitor cells as well as differentiation of early B lymphoid progenitors (10409713). In the mammalian retina, FIZ1 interacts with NRL (Neural-Retina Leucine-zipper), an essential transcriptional activator of rod photoreceptor-specific genes, and the concentration of FIZ1 in the retina will increase during photoreceptor terminal maturation (18854042).