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ChromoTek FITC Plus-conjugated MSLN VHH

FITC-msln targets MSLN in FC and IF applications and shows reactivity with Human samples.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

FC, IF

Conjugate

FITC Plus Fluorescent dye

Cat no : FITC-msln

Synonyms

MSLN-Nanobody, MSLN-Single domain antibody (sdAb), CAK1 antigen, pre-pro-megakaryocyte-potentiating factor, MPF, MSLN, Mesothelin

Validation Data Gallery

  • Left: 0.5x10^6 mixed HeLa and PC-3 cells were surface stained with 0.5ug FITC Plus conjugated MSLN VHH (FITC-msln). Right: 0.5x10^6 HeLa (red) or PC-3 (black dashed) cells were surface stained with FITC Plus conjugated MSLN VHH (FITC-msln) or unstained (blue).

    Left: 0.5x10^6 mixed HeLa and PC-3 cells were surface stained with 0.5ug FITC Plus conjugated MSLN VHH (FITC-msln). Right: 0.5x10^6 HeLa (red) or PC-3 (black dashed) cells were surface stained with FITC Plus conjugated MSLN VHH (FITC-msln) or unstained (blue).

  • HeLa cells transfected with human Mesothelin were live immunostained with FITC Plus conjugated MSLN VHH (1:500, green). Nuclei were stained with DAPI (blue or cyan) after cell fixation. Epifluorescence images were acquired with a 20x objective and post-processed. Comment: Immunostaining with FITC Plus conjugated MSLN VHH can be performed live or after formaldehyde fixation.

    HeLa cells transfected with human Mesothelin were live immunostained with FITC Plus conjugated MSLN VHH (1:500, green). Nuclei were stained with DAPI (blue or cyan) after cell fixation. Epifluorescence images were acquired with a 20x objective and post-processed. Comment: Immunostaining with FITC Plus conjugated MSLN VHH can be performed live or after formaldehyde fixation.

  • Left: 0.5x10^6 mixed HeLa and PC-3 cells were surface stained with 0.125ug FITC Plus conjugated Mesothelin VHH (FITC-msln). Right: 0.5x10^6 HeLa (red) or PC-3 (black dashed) cells were surface stained with 0.125ug FITC Plus conjugated Mesothelin VHH (FITC-msln) or unstained (blue).

    Left: 0.5x10^6 mixed HeLa and PC-3 cells were surface stained with 0.125ug FITC Plus conjugated Mesothelin VHH (FITC-msln). Right: 0.5x10^6 HeLa (red) or PC-3 (black dashed) cells were surface stained with 0.125ug FITC Plus conjugated Mesothelin VHH (FITC-msln) or unstained (blue).

Product Information

FITC-msln targets MSLN in FC and IF applications and shows reactivity with Human samples.

ApplicationsFC, IF
ReactivityHuman
ConjugateFITC Plus Fluorescent dye
TypeNanobody
ClassRecombinant
HostAlpaca
AffinityPicomolar range, below the assay limit (biolayer interferometry)
Molecular Weight15.710 kDa
FormLiquid
Excitation/Emission Maxima Wavelengths495 nm / 524 nm
RRIDAB_3101941
Storage Buffer500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide
Storage ConditionStore at -20°C
BackgroundMesothelin (MSLN) is a 40kDa GPI-anchored surface glycoprotein that is expressed on pleural and peritoneal mesothelial cells, but not in other non-neoplastic tissues. The mesothelin gene encodes a 69-71kDa precursor protein that is processed to a 40kDa membrane-bound protein termed mesothelin and a 31kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Mesothelin is a tumor differentiation antigen present at low levels on a restricted set of normal adult tissues (e.g. mesothelium), and is expressed at high levels in mesotheliomas, as well as ovarian, pancreatic, and lung cancers. Mesothelin may be a new target for immunotherapy (PMID: 20922056, 18691948, 15217923).