ChromoTek FITC Plus-conjugated CTLA4 VHH

FITC-ctla targets CTLA4 in FC and IF applications and shows reactivity with Human samples.

Host/Type

Alpaca VHH

Reactivity

Human

Applications

FC, IF

Conjugate

FITC Plus Fluorescent dye

Cat no : FITC-ctl

Synonyms

CTLA4-Nanobody, CTLA4-Single domain antibody (sdAb), CD152, Cytotoxic T-Lymphocyte-associated antigen 4, CTLA4, CTLA-4

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Validation Data Gallery

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  1X10^6 HEK CTLA4 transient transfected cells (red) and HEK parental cells were surface stained with 0.5 µg FITC Plus conjugated-CTLA4 VHH (FITC-ctl). Cells were not fixed.

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  Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 1ug FITC Plus conjugated CTLA-4 VHH (FITC-ctl) and CL405 CD3 (CL405-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 1ug FITC Plus conjugated CTLA-4 VHH (FITC-ctl) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 1ug FITC Plus conjugated CTLA-4 VHH (FITC-ctl) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were fixed and permeabilized using Transfripction Factor Staining Buffer Kit (PF00011). Lymphocytes were gated.

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  Left: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug FITC Plus conjugated CTLA-4 VHH (FITC-ctl) and CL750 CD3 (CL750-65151). Right: 1x10^6 PHA-treated (3d) human PBMCs were surface stained with 0.5ug FITC Plus conjugated CTLA-4 VHH (FITC-ctl) (red) or unstained (blue). 1X10^6 untreated human PBMCs were surface stained with 0.5ug FITC Plus conjugated CTLA-4 VHH (FITC-ctl) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were not fixed. Lymphocytes were gated.

• 

  1x10^6 PHA-treated (3d) human PBMCs were surface stained with CL405 CD3 (CL405-65151) then fixed and permeabilized and stained with 1ug FITC Plus conjugated CTLA-4 VHH (FITC-ctl). Right: 1x10^6 PHA-treated (3d) human PBMCs were fixed and permeabilized then stained with 1ug FITC Plus conjugated CTLA-4 VHH (FITC-ctl) (red) or unstained (blue). 1X10^6 untreated human PBMCs were fixed and permeabilized then stained with 1ug FITC Plus conjugated CTLA-4 VHH (FITC-ctl) (black dashed). Cells were treated with FC Receptor Block prior to staining. Cells were fixed and permeabilized using Transfripction Factor Staining Buffer Kit (PF00011). Lymphocytes were gated.

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  HeLa cells transfected with human CTLA-4 were immunostained with FITC Plus conjugated-CTLA4 VHH (FITC-ctl) (1:500, green). Nuclei were stained with DAPI (blue). Epifluorescence images were acquired with a 40x objective and post-processed. Comment: Immunostaining with FITC-ctl can be performed live or after formaldehyde fixation.

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Product Information

FITC-ctla targets CTLA4 in FC and IF applications and shows reactivity with Human samples.

• Applications: FC, IF
• Reactivity: Human
• Conjugate: FITC Plus Fluorescent dye
• Type: Nanobody
• Class: Recombinant
• Host: Alpaca
• Affinity: 22 nM
• Molecular Weight: 14.005 kDa
• Form: Liquid
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• RRID: AB_3101937
• Storage Buffer: 500 mM NaCl, 10 mM HEPES pH 7.0, 5 mM EDTA, 0.09% sodium azide
• Storage Condition: Store at -20°C
• Background: CTLA-4, also known as CD152, belonging to the immunoglobulin superfamily, is primarily found on activated T cells and regulatory T cells (Tregs). CTLA-4 is closely related to the T-cell costimulatory CD28, and both molecules bind to B7-1 and B7-2 on antigen-presenting cells. CTLA-4 acts as a negative regulatory molecule of T-cell responses. Besides the full-length transmembrane form, CTLA-4 also exists in a truncated soluble form (sCTLA-4).