FITC Monoclonal antibody

FITC Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

chemical compound, fitc

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C10A1

Cat no : 68132-1-Ig

Synonyms

Fluorescein isothiocyanate



Tested Applications

Positive WB detected inFITC-TDP43, FITC-Ag1231, BSA
Positive ELISA detected inFITC conjugated antibody

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Enzyme-linked Immunosorbent Assay (ELISA)ELISA :
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68132-1-Ig targets FITC in WB, ELISA applications and shows reactivity with chemical compound, fitc samples.

Tested Reactivity chemical compound, fitc
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Compound
Full Name FITC
Gene Symbol
Gene ID (NCBI)
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This mouse monoclonal antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.

Protocols

Product Specific Protocols
WB protocol for FITC antibody 68132-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols