FITC Monoclonal antibody

FITC Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

chemical compound, fitc

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C10A1

Cat no : 68132-1-Ig

Synonyms

Fluorescein isothiocyanate

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Validation Data Gallery

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  WB analysis using 68132-1-Ig

  WB result of FITC antibody (68132-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with FITC conjugated protein and un-conjugated protein as negative control. Antibody to TDP-43 as the positive control to target protein.

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  WB analysis of BSA using 68132-1-Ig

  WB results of FITC antibody (68132-1-Ig, 1:10000) with BSA and FITC conjugated BSA.

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  ELISA experiment of FITC conjugated antibody using 68132-1-Ig

  Indirect ELISA and competitive ELISA results show that this antibody is specific to FITC. Indirect ELISA was performed by coating FITC conjugated BSA at 0ng/well followed by blocking with 1% BSA. Serial diluted primary antibody was added to the plates and incubated at 37℃. HRP-goat anti-mouse was used for detection (red curve, refer to top X-right Y axis). Competitive ELISA was performed similarly except that different concentration of FITC (active group been quenched) was mixed in 3.3ng/mL primary antibody (blue curve, refere to bottom X-left Y axis).

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Tested Applications

• Positive WB detected in: FITC-TDP43, FITC-Ag1231, BSA
• Positive ELISA detected in: FITC conjugated antibody

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Enzyme-linked Immunosorbent Assay (ELISA): ELISA :

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68132-1-Ig targets FITC in WB, ELISA applications and shows reactivity with chemical compound, fitc samples.

• Tested Reactivity: chemical compound, fitc
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Compound
• Full Name: FITC
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications. It is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. It is typically available as a mixture of isomers, fluorescein 5-isothiocyanate (5-FITC) and fluorescein 6-isothiocyanate (6-FITC). FITC is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. CAS number of FITC: 27072-45-3 (mixture); 3326-32-7, 5-isomer; 18861-78-4 (6-isomer). This mouse monoclonal antibody detects the FITC only. This antibody can be coated in the plate to capture the FITC conjugated protein or antibody.