Tested Applications
Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
Positive FC (Intra) detected in | A549 cells, U2OS cells |
Recommended dilution
Application | Dilution |
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Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
83863-3-RR targets FIP1L1 in IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | FIP1L1 fusion protein Ag2921 Predict reactive species |
Full Name | FIP1 like 1 (S. cerevisiae) |
Calculated Molecular Weight | 594 aa, 66 kDa |
GenBank Accession Number | BC017724 |
Gene Symbol | FIP1L1 |
Gene ID (NCBI) | 81608 |
RRID | AB_3671449 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q6UN15 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).
Protocols
Product Specific Protocols | |
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IHC protocol for FIP1L1 antibody 83863-3-RR | Download protocol |
IF protocol for FIP1L1 antibody 83863-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |