FIP1L1 Polyclonal antibody

FIP1L1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12276-1-AP

Synonyms

RHE, Pre-mRNA 3'-end-processing factor FIP1, FIP1-like 1 protein, FIP1

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Validation Data Gallery

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  WB analysis of K-562 using 12276-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12276-1-AP (FIP1L1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 12276-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12276-1-AP (FIP1L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 12276-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12276-1-AP (FIP1L1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12276-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12276-1-AP (FIP1L1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 12276-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FIP1L1 antibody (12276-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of MCF-7 using 12276-1-AP

  Immunofluorescent analysis of MCF-7 cells, using FIP1L1 antibody 12276-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: human colon cancer tissue, human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12276-1-AP targets FIP1L1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FIP1L1 fusion protein Ag2921
• Full Name: FIP1 like 1 (S. cerevisiae)
• Calculated Molecular Weight: 594 aa, 66 kDa
• Observed Molecular Weight: 67 kDa
• GenBank Accession Number: BC017724
• Gene Symbol: FIP1L1
• Gene ID (NCBI): 81608
• RRID: AB_2231572
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FIP1L1, also known as FIP1, encodes a subunit of the CPSF (cleavage and polyadenylation specificity factor) complex that polyadenylates the 3' end of mRNA precursors. The FIP1L1-PDGFRA fusion gene encodes a constitutively activated tyrosine kinase that links the first 233 amino acids of FIP1L1 to the last 523 amino acids of PDGFRA. This gene fusion and chromosomal deletion causes some forms of idiopathic hypereosinophilic syndrome (HES)( PMID: 12660384).