FGL2 Polyclonal antibody

FGL2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 11827-1-AP

Synonyms

Fibroleukin, Fibrinogen-like protein 2, Fibrinogen like protein 2, fibrinogen like 2, FGL-2

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Validation Data Gallery

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  WB analysis of human ileum using 11827-1-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 11827-1-AP (FGL2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis using 11827-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11827-1-AP (FGL2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 11827-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 11827-1-AP (FGL2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 11827-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11827-1-AP (FGL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 11827-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11827-1-AP (FGL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 11827-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11827-1-AP (FGL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 11827-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11827-1-AP (FGL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11827-1-AP

  Immunofluorescent analysis of HepG2 cells, using FGL2 antibody 11827-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: mouse colon tissue, human ileum tissue, mouse thymus tissue, rat thymus tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below
• IHC: See 6 publications below
• IF: See 1 publications below

Product Information

11827-1-AP targets FGL2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGL2 fusion protein Ag2395
• Full Name: fibrinogen-like 2
• Calculated Molecular Weight: 50 kDa
• Observed Molecular Weight: 70 kDa, 50-60 kDa
• GenBank Accession Number: BC017813
• Gene Symbol: FGL2
• Gene ID (NCBI): 10875
• RRID: AB_2103947
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGL2 (fibrinogen like protein 2) is a 70 kDa glycoprotein that belongs to the fibrinogen-related superfamily of proteins which are involved in coagulation, cell adhesion, and transendothelial migration. FGL2 is an important immune regulator of both innate and adaptive response. It is present on the surface of macrophages and endothelial cells, and can be constitutively secreted by CD4+ CD8+ T cell. FGL2 forms a tetrameric complex which is stabilized by interchain disulfide bonds and it exerts immunosuppressive effects on both T cell proliferation and dendritic cell maturation. The protein may play a role in physiologic functions at mucosal sites.

Publications

Species	Application	Title
human	IHC	Nat Commun; Cerebrospinal fluid lipocalin 2 as a novel biomarker for the differential diagnosis of vascular dementia.; Authors - Franc Llorens
human	IHC	Int Immunopharmacol; Identification of integrative molecular and clinical profiles of Fibrinogen-like protein 2 in gliomas using 1323 samples.; Authors - Zhizhen Song
mouse	IHC	Mol Immunol; IL-33 protects murine viral fulminant hepatitis by targeting coagulation hallmark protein FGL2/fibroleukin expression.; Authors - Haijing Yu
human	WB	Oncoimmunology; Integrative analysis identifies an immune-relevant epigenetic signature for prognostication of non-G-CIMP glioblastomas.; Authors - Anan Yin
mouse	WB	Cell Metab; Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain; Authors - Hai-Yan Zhou
mouse	IHC,IF	Phytomedicine; Cuyun Recipe ameliorates pregnancy loss by regulating macrophage polarization and hypercoagulable state during the peri-implantation period in an ovarian hyperstimulation mouse model; Authors - Wenxin Zhu