FGL1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FGL1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

19

Cat no : 16000-1-AP

Synonyms

FGL1, fibrinogen like 1, Fibrinogen like protein 1, Hepassocin, HFREP 1, HFREP1, HP 041, HPS, LFIRE 1, LFIRE1

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Validation Data Gallery

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  WB analysis using 16000-1-AP

  Human placenta tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16000-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 16000-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 16000-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 16000-1-AP (FGL1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 16000-1-AP

  IP result of anti-FGL1 (IP:16000-1-AP, 4ug; Detection:16000-1-AP 1:500) with HeLa cells lysate 2000ug.

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  IHC staining of human liver cancer using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human hepatocirrhosis using 16000-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 16000-1-AP (FGL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human placenta tissue, human liver tissue, HepG2 cells, mouse thymus tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue, human hepatocirrhosis tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 11 publications below
• IF: See 2 publications below

Product Information

16000-1-AP targets FGL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGL1 fusion protein Ag8581
• Full Name: fibrinogen-like 1
• Calculated Molecular Weight: 312 aa, 36 kDa
• Observed Molecular Weight: 34 kDa, 68 kDa
• GenBank Accession Number: BC007047
• Gene Symbol: FGL1
• Gene ID (NCBI): 2267
• RRID: AB_2103936
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGL1 is a 68 kDa protein that comprises two disulfide linked 34 kDa homodimers. FGL1 is a predominantly liver expressed protein that has been implicated as both a hepatoprotectant and a hepatocyte mitogen. FGL1 expression is decreased in hepatocellular carcinoma (HCC), and it may play a role in the development of hepatocellular carcinomas.

Publications

Species	Application	Title
human	WB,IHC	Transl Res; Role of placental fibrinogen-like protein 1 in gestational diabetes.; Authors - Lin Kang
mouse	IHC	Front Immunol; Fibrinogen-like protein 1 promotes liver-resident memory T-cell exhaustion in hepatocellular carcinoma; Authors - Changjie Yang
mouse	WB	Diabetologia; PMID: 27221093; Authors - Hung-Tsung Wu ; KD Validated
mouse	WB,IHC	J Nanobiotechnology; Regulating the immunosuppressive tumor microenvironment to enhance breast cancer immunotherapy using pH-responsive hybrid membrane-coated nanoparticles.; Authors - Chunai Gong
human	WB	Int J Mol Sci; Fibrinogen-Like Protein 1 Modulates Sorafenib Resistance in Human Hepatocellular Carcinoma Cells.; Authors - Yeonghoon Son
human,mouse	WB	J Clin Endocrinol Metab; The Hepatic Protection Effects of Hepassocin in Hyperglycemic Crisis.; Authors - Horng-Yih Ou