FGFR3 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 66954-1-Ig (same clone as 66954-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66954-1-Ig (same clone as 66954-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  WB analysis of L-929 using 66954-1-Ig (same clone as 66954-1-PBS)

  L-929 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66954-1-Ig (same clone as 66954-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66954-1-Ig (same clone as 66954-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66954-1-Ig (FGFR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGFR3 antibody (66954-1-Ig, Clone: 1F3G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 66954-1-Ig (same clone as 66954-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FGFR3 antibody (66954-1-Ig, Clone: 1F3G1 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66954-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP50578-1

  Cytometric bead array standard curve of MP50578-1, FGFR3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66954-1-PBS. Detection antibody: 66954-2-PBS. Standard:Ag26290. Range: 1.563-200 ng/mL.

• Featured Product
• KD/KO Validated

FGFR3 Monoclonal Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA

Cat No. 66954-1-PBS

Clone No.1F3G1

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Indirect ELISA

Synonyms

JTK4, HSFGFR3EX, FGFR 3, CEK2, CD333

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Product Information

66954-1-PBS targets FGFR3 as part of a matched antibody pair:

MP50578-1: 66954-1-PBS capture and 66954-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FGFR3 fusion protein Ag26290
• Full Name: fibroblast growth factor receptor 3
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 125-135 kDa
• GenBank Accession Number: NM_000142
• Gene Symbol: FGFR3
• Gene ID (NCBI): 2261
• RRID: AB_2882277
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Fibroblast growth factors (FGFs) are polypeptide growth factors involved in a variety of activities including mitogenesis, angiogenesis, and wound healing (PMID: 1847508). The human FGF receptor family, a subfamily of receptor tyrosine kinases (RTKs), comprises of four family members-FGFR1, FGFR2, FGFR3, and FGFR4 (PMID: 23900974). Each receptor contains an extracellular domain with either two or three immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tyrosine kinase domain. FGFR3 binds acidic and basic fibroblast GH and plays a role in bone development and maintenance. Mutations in the FGFR3 gene lead to craniosynostosis and multiple types of skeletal dysplasia. Due to frequent mutations in certain cancers, the FGFR3 gene has also been associated with tumor progression.