FGF8 Polyclonal antibody

FGF8 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Reviews (1)

Cat no : 20711-1-AP

Synonyms

AIGF, Androgen induced growth factor, FGF 8, FGF8, Fibroblast growth factor 8, HBGF 8, KAL6

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Validation Data Gallery

• 

  WB analysis using 20711-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 20711-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20711-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20711-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20711-1-AP (FGF8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse liver tissue, L02 cells, HeLa cells, rat liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

20711-1-AP targets FGF8 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: fibroblast growth factor 8 (androgen-induced)
• Calculated Molecular Weight: 27 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: NM_006119
• Gene Symbol: FGF8
• Gene ID (NCBI): 2253
• RRID: AB_10733225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGF8, also named as AIGF and HBGF-8, belongs to the heparin-binding growth factors family. FGF8 stimulates growth of the cells in an autocrine manner. It mediates hormonal action on the growth of cancer cells. Defects in FGF8 are the cause of Kallmann syndrome type 6 (KAL6). Defects in FGF8 are a cause of idiopathic hypogonadotropic hypogonadism (IHH). The antibody is specific to FGF8.

Publications

Species	Application	Title
human	WB,IHC	Carcinogenesis; The oncoprotein HBXIP enhances angiogenesis and growth of breast cancer through modulating FGF8 and VEGF.; Authors - Fabao Liu
human	IF	Oncoimmunology; Immunoreactivity against fibroblast growth factor 8 in alveolar rhabdomyosarcoma patients and its involvement in tumor aggressiveness.; Authors - Elena Poli
human	IHC	Oncol Lett; Systematic analysis of expression profiles and prognostic significance of the FGF gene family in pancreatic adenocarcinoma; Authors - Yu-Xin Chen

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paolo (Verified Customer) (12-12-2019)

It works fine in wb, in IF differences in FGF8 intracellular localization were observed according to the fixative solution used

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1/500