FGF5 Polyclonal antibody

• Featured Product
• KD/KO Validated

FGF5 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 18171-1-AP

Synonyms

FGF 5, FGF5, fibroblast growth factor 5, HBGF 5, Smag 82

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Validation Data Gallery

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  IHC staining of human stomach using 18171-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18171-1-AP (FGF5 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 18171-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 18171-1-AP (FGF5 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human placenta using 18171-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18171-1-AP (FGF5 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 18171-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18171-1-AP (FGF5 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human malignant melanoma using 18171-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18171-1-AP (FGF5 antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human malignant melanoma using 18171-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 18171-1-AP (FGF5 antibody) at dilution of 1:400 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human stomach tissue, human placenta tissue, human malignant melanoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IHC: See 6 publications below
• IF: See 3 publications below

Product Information

The immunogen of 18171-1-AP is FGF5 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, goat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGF5 fusion protein Ag12824
• Full Name: fibroblast growth factor 5
• Calculated Molecular Weight: 268 aa, 30 kDa
• GenBank Accession Number: BC074858
• Gene Symbol: FGF5
• Gene ID (NCBI): 2250
• RRID: AB_2878511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rabbit		Mol Ther Nucleic Acids; CRISPR Start-Loss: A Novel and Practical Alternative for Gene Silencing through Base-Editing-Induced Start Codon Mutations.; Authors - Siyu Chen ; KO Validated
mouse		iScience; Commercial dishes with gelatin-free microstructured inserts for elongated stem cell self-renewal and pluripotency; Authors - Qian Ban
goat		Int J Biol Sci; Generation of VEGF knock-in Cashmere goat via the CRISPR/Cas9 system.; Authors - Xiao Hu
mouse	IHC	Front Pharmacol; Suppression of FGF5 and FGF18 Expression by Cholesterol-Modified siRNAs Promotes Hair Growth in Mice.; Authors - Jungang Zhao
mouse	IF	Commun Biol; Testicular somatic cell-like cells derived from embryonic stem cells induce differentiation of epiblasts into germ cells.; Authors - Holly Rore
mouse	IHC	Genes (Basel); CRISPR/Cas9-mediated Disruption of Fibroblast Growth Factor 5 in Rabbits Results in a Systemic Long Hair Phenotype by Prolonging Anagen.; Authors - Yuxin Xu