FGF17 Polyclonal antibody

FGF17 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25314-1-AP

Synonyms

FGF 13, FGF 17, FGF17, fibroblast growth factor 17

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 25314-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25314-1-AP (FGF17 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 25314-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 25314-1-AP (FGF17 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IF Staining of NIH/3T3 using 25314-1-AP

  Immunofluorescent analysis of NIH/3T3 cells using 25314-1-AP (FGF17 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: MCF-7 cells, PC-3 cells
• Positive IF/ICC detected in: NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

The immunogen of 25314-1-AP is FGF17 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGF17 fusion protein Ag17807
• Full Name: fibroblast growth factor 17
• Calculated Molecular Weight: 216 aa, 25 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC105131
• Gene Symbol: FGF17
• Gene ID (NCBI): 8822
• RRID: AB_2880025
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
	IF	Cell; Ex utero monkey embryogenesis from blastocyst to early organogenesis; Authors - Yandong Gong