FGF12 Monoclonal antibody

FGF12 Monoclonal Antibody for WB, ELISA, FC (Intra)

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, ELISA, FC (Intra)

Conjugate

Unconjugated

CloneNo.

2F11A10

Cat no : 60152-1-Ig

Synonyms

FGF 12, FGF12, FGF12B, FHF 1, FHF1, fibroblast growth factor 12, Myocyte activating factor

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Validation Data Gallery

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  WB analysis of human brain using 60152-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 60152-1-Ig (FGF12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 60152-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60152-1-Ig (FGF12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 60152-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60152-1-Ig (FGF12 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 60152-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug FGF12 Monoclonal antibody (60152-1-Ig, Clone:2F11A10) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2b isotype control Mouse McAb (66360-3-Ig, Clone: 11B8C4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: mouse brain tissue, human brain tissue, rat brain tissue
• Positive FC (Intra) detected in: HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

60152-1-Ig targets FGF12 in WB, ELISA, FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FGF12 fusion protein Ag5046
• Full Name: fibroblast growth factor 12
• Calculated Molecular Weight: 181 aa, 20 kDa
• Observed Molecular Weight: 20 kDa
• GenBank Accession Number: BC022524
• Gene Symbol: FGF12
• Gene ID (NCBI): 2257
• RRID: AB_10644325
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGF12 is a member of the fibroblast growth factor (FGF) family. FGF family members play important roles in embryogenesis, angiogenesis, and wound repair. FGF12 lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. FGF12 plays an intracellular role in the inhibition of radiation-induced apoptosis. FGF12 is expressed abundantly in developing and adult nervous systems; therefore, FGF12 was thought to be related to nervous system development and function.