FGF12 Polyclonal antibody

• Featured Product
• KD/KO Validated

FGF12 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 13784-1-AP

Synonyms

FGF 12, FGF12, FGF12B, FHF 1, FHF1, Myocyte activating factor

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Validation Data Gallery

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  WB analysis using 13784-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13784-1-AP (FGF12 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13784-1-AP

  IP result of anti-FGF12 (IP:13784-1-AP, 4ug; Detection:13784-1-AP 1:6000) with mouse brain tissue lysate 1280 ug.

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  IHC staining of mouse brain using 13784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13784-1-AP (FGF12 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse brain using 13784-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13784-1-AP (FGF12 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

13784-1-AP targets FGF12 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGF12 fusion protein Ag4797
• Full Name: fibroblast growth factor 12
• Calculated Molecular Weight: 181 aa, 20 kDa
• Observed Molecular Weight: 20-22 kDa
• GenBank Accession Number: BC022524
• Gene Symbol: FGF12
• Gene ID (NCBI): 2257
• RRID: AB_2103928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGF12 is a member of the FGF family. FGF family members play important roles in embryogenesis, angiogenesis, and wound repair. FGF12 lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. FGF12 plays an intracellular role in the inhibition of radiation-induced apoptosis. FGF12 is expressed abundantly in developing and adult nervous systems; therefore, FGF12 was thought to be related to nervous system development and function.

Publications

Species	Application	Title
rat	IF	Neurobiol Dis; Decreased expression of synaptic genes in the vestibular ganglion of rodents following subchronic ototoxic stress; Authors - Erin A Greguske
human	WB	Epilepsia; Early onset epilepsy and sudden unexpected death in epilepsy with cardiac arrhythmia in mice carrying the early infantile epileptic encephalopathy 47 gain-of-function FHF1(FGF12) missense mutation.; Authors - Jana Velíšková
mouse	WB,IF	Sci Rep; Fibroblast growth factor 12 is expressed in spiral and vestibular ganglia and necessary for auditory and equilibrium function.; Authors - Yukiko Hanada ; KO Validated
human	IHC	Heliyon; Identification of cancer-associated fibroblast-related Ectodysplasin-A as a novel indicator for prognosis and immune response in gastric cancer; Authors - Ya Zhang
mouse	WB	Nat Commun; Proximity analysis of native proteomes reveals phenotypic modifiers in a mouse model of autism and related neurodevelopmental conditions; Authors - Yudong Gao