FGF-21 Recombinant antibody

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  IF Staining of RAW 264.7 using 82706-6-RR

  Immunofluorescent analysis of (4% PFA) fixed LPS and Brefeldin A treated RAW 264.7 cells using FGF-21 antibody (82706-6-RR, Clone: 240221D10 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

FGF-21 {ptg:Brand} Recombinant Antibody for IF/ICC, ELISA

Cat No. 82706-6-RR

Clone No.240221D10

Host / Isotype

Rabbit / IgG

Reactivity

Mouse

Applications

IF/ICC, ELISA

Synonyms

Fgf21, fibroblast growth factor 21

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Tested Applications

• Positive IF/ICC detected in: LPS and Brefeldin A treated RAW 264.7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

82706-6-RR targets FGF-21 in IF/ICC, ELISA applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: fibroblast growth factor 21
• Calculated Molecular Weight: 23kd
• GenBank Accession Number: NM_020013
• Gene Symbol: FGF-21
• Gene ID (NCBI): 56636
• RRID: AB_3670534
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9JJN1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor 21 (FGF21), a member of the FGF superfamily, has emerged as an important metabolic regulator. FGF21 is produced in the liver, adipocytes and skeletal muscle and regulates glucose, energy and lipid metabolism. FGF21 is a stress-responsive hepatokine which is induced in the liver as a response to injury, regeneration, hepatocarcinogenesis and during kidney dysfunction. FGF21 is primarily metabolized in the kidney and renal secretion is the major route of FGF21 elimination. Its plasma levels increase according to the decline in renal function in patients with chronic and acute renal dysfunction.