FGF-2 Polyclonal antibody

FGF-2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 30333-1-AP

Synonyms

Basic fibroblast growth factor, BFGF, FGF2, FGF-2, FGFB, HBGF 2

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Validation Data Gallery

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  WB analysis using 30333-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30333-1-AP (FGF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of SKOV-3 using 30333-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using FGF-2 antibody (30333-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: K-562 cells, SKOV-3 cells, U-87 MG cells
• Positive IF/ICC detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

30333-1-AP targets FGF-2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGF-2 fusion protein Ag28653
• Full Name: fibroblast growth factor 2 (basic)
• Calculated Molecular Weight: 31 aa
• Observed Molecular Weight: 21 kDa，23 kDa
• GenBank Accession Number: NM_002006
• Gene Symbol: FGF2
• Gene ID (NCBI): 2247
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibroblast growth factor-2 (FGF-2), also referred to as basic FGF, belongs to a family of growth factors that stimulate proliferation of cells of mesenchymal, epithelial and neuroectodermal origin. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in at least five different isoforms with distinct properties.

Publications

Species	Application	Title
mouse	IHC	Bioengineering (Basel); Shark Cartilage-Derived Anti-Angiogenic Peptide Inhibits Corneal Neovascularization; Authors - Yunxian Li