FGD3 Polyclonal antibody

• Featured Product
• KD/KO Validated

FGD3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 20347-1-AP

Synonyms

Zinc finger FYVE domain-containing protein 5, ZFYVE5, FYVE, RhoGEF and PH domain-containing protein 3

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Validation Data Gallery

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  WB analysis using 20347-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20347-1-AP (FGD3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 20347-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 20347-1-AP (FGD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20347-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20347-1-AP (FGD3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 20347-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20347-1-AP (FGD3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 20347-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 20347-1-AP (FGD3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 20347-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 20347-1-AP (FGD3 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, K-562 cells, HEK-293T cells, HeLa cells, A549 cells
• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

20347-1-AP targets FGD3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGD3 fusion protein Ag14155
• Full Name: FYVE, RhoGEF and PH domain containing 3
• Calculated Molecular Weight: 725 aa, 79 kDa
• Observed Molecular Weight: 79 kDa
• GenBank Accession Number: BC032429
• Gene Symbol: FGD3
• Gene ID (NCBI): 89846
• RRID: AB_10693616
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC	Oncogene; FGD3 binds with HSF4 to suppress p65 expression and inhibit pancreatic cancer progression.; Authors - Feng Guo ; KD Validated