FGD1 Recombinant antibody

FGD1 Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

242314C3

Cat no : 84783-2-RR

Synonyms

Zinc finger FYVE domain-containing protein 3, ZFYVE3, Rho/Rac GEF, FYVE, RhoGEF and PH domain-containing protein 1, FGDY

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Validation Data Gallery

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  WB analysis using 84783-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84783-2-RR (FGD1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84783-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84783-2-RR against Human FGD1 were performed. The affinity constant is below 1 pM.

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Tested Applications

• Positive WB detected in: SK-N-SH cells, U-87 MG cells, BxPC-3 cells, U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84783-2-RR targets FGD1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FGD1 fusion protein Ag3861
• Full Name: FYVE, RhoGEF and PH domain containing 1
• Calculated Molecular Weight: 901 aa, 91 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC034530
• Gene Symbol: FGD1
• Gene ID (NCBI): 2245
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGD1 is also named as FGDY (Faciogenital dysplasia 1 protein), ZFYVE3 (Zinc finger FYVE domain-containing protein 3), Rho/Rac guanine nucleotide exchange factor FGD1 (Rho/Rac GEF), FYVE, RhoGEF and PH domain-containing protein 1. FGD1 activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. And FGD1 plays a role in regulating the actin cytoskeleton and cell shape. (PMID: 8969170). FGD1 was markedly overexpressed and might be a prognostic biomarker in osteosarcoma patient specimens (PMID: 32194840). FGD1 is specific for the Rho GTPase cell division cycle 42 (CDC42) (PMID: 22854039).