FGD1 Polyclonal antibody

FGD1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 13141-1-AP

Synonyms

Zinc finger FYVE domain-containing protein 3, ZFYVE3, Rho/Rac GEF, FYVE, RhoGEF and PH domain-containing protein 1, FGDY

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Validation Data Gallery

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  WB analysis using 13141-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13141-1-AP (FGD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 13141-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13141-1-AP (FGD1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of SK-N-SH using 13141-1-AP

  IP result of anti-FGD1 (IP:13141-1-AP, 4ug; Detection:13141-1-AP 1:600) with SK-N-SH cells lysate 1160 ug.

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  IHC staining of mouse kidney using 13141-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 13141-1-AP (FGD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 13141-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using FGD1 antibody (13141-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, SK-N-SH cells, U2OS cells
• Positive IP detected in: SK-N-SH cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13141-1-AP targets FGD1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FGD1 fusion protein Ag3861
• Full Name: FYVE, RhoGEF and PH domain containing 1
• Calculated Molecular Weight: 901 aa, 91 kDa
• Observed Molecular Weight: 130 kDa
• GenBank Accession Number: BC034530
• Gene Symbol: FGD1
• Gene ID (NCBI): 2245
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FGD1 is also named as FGDY (Faciogenital dysplasia 1 protein), ZFYVE3 (Zinc finger FYVE domain-containing protein 3), Rho/Rac guanine nucleotide exchange factor FGD1 (Rho/Rac GEF), FYVE, RhoGEF and PH domain-containing protein 1. FGD1 activates CDC42, a member of the Ras-like family of Rho- and Rac proteins, by exchanging bound GDP for free GTP. And FGD1 plays a role in regulating the actin cytoskeleton and cell shape. (PMID: 8969170). FGD1 was markedly overexpressed and might be a prognostic biomarker in osteosarcoma patient specimens (PMID: 32194840). FGD1 is specific for the Rho GTPase cell division cycle 42 (CDC42) (PMID: 22854039).