FFAR3 Monoclonal antibody

FFAR3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (4)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

11

CloneNo.

1D10B7

Reviews (1)

Cat no : 66811-1-Ig

Synonyms

FFA3R, FFAR3, free fatty acid receptor 3, G protein coupled receptor 41, GPR41

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Validation Data Gallery

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  WB analysis using 66811-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human adipose using 66811-1-Ig

  human adipose tissue were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 66811-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66811-1-Ig (FFAR3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 66811-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66811-1-Ig (FFAR3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of A549 using 66811-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using 66811-1-Ig (FFAR3 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, human adipose tissue, HT-29 cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IF: See 4 publications below

Product Information

66811-1-Ig targets FFAR3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: FFAR3 fusion protein Ag28025
• Full Name: free fatty acid receptor 3
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 40-48 kDa
• GenBank Accession Number: BC035657
• Gene Symbol: FFAR3
• Gene ID (NCBI): 2865
• RRID: AB_2882154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FFAR3 (Free fatty acid receptors 3) also known as GPR41, with another GPR43 protein, both are Gai-coupled receptor activated by short-chain fatty acids (SCFAs) such as acetate, propionate, and butyrate (PMID:26870043). GPR41 protein is translated from the bicistronic mRNA encoding GPR40 and GPR41, where an internal ribosome entry site (IRES) is utilized for the GPR41 coding sequence downstream of GPR40 (PMID:22493486). GPR41 is expressed in adipose tissue, gut, and the peripheral nervous system, and it is involved in SCFA-dependent energy regulation(PMID: 24904531).

Publications

Species	Application	Title
		Cell Host Microbe; Roseburia intestinalis-derived butyrate alleviates neuropathic pain; Authors - Yanjun Jiang
human	IF	Front Immunol; Natural fish oil improves the differentiation and maturation of oligodendrocyte precursor cells to oligodendrocytes in vitro after interaction with the blood-brain barrier; Authors - Paweł Piatek
human	WB,IF	Front Microbiol; Gut microbiome and serum short-chain fatty acids are associated with responses to chemo- or targeted therapies in Chinese patients with lung cancer; Authors - Huan-Huan Chen
human	WB	Gut Microbes; Butyrate reduces adherent-invasive E. coli-evoked disruption of epithelial mitochondrial morphology and barrier function: involvement of free fatty acid receptor 3; Authors - Samira A Hamed
human	WB	Gut Microbes; Sialic acid-based probiotic intervention in lactating mothers improves the neonatal gut microbiota and immune responses by regulating sialylated milk oligosaccharide synthesis via the gut-breast axis; Authors - Yushuang Wang
rat	WB	Food Funct; Improving insulin resistance by sulforaphane via activating the Bacteroides and Lactobacillus SCFAs-GPR-GLP1 signal axis; Authors - Sicong Tian

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christin (Verified Customer) (01-26-2025)

Great antibody for western blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1500 in milk
• Cell Tissue Type: Human iPSC-derived macrophages and microglia