FEZ2 Recombinant antibody, PBS Only

FEZ2 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240248B10

Cat no : 83407-7-PBS

Synonyms

Zygin-2, Zygin II, Zygin 2, HUM3CL, Fasciculation and elongation protein zeta-2

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Validation Data Gallery

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  WB analysis using 83407-7-RR (same clone as 83407-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83407-7-RR (FEZ2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83407-7-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 83407-7-RR (same clone as 83407-7-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83407-7-RR (FEZ2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83407-7-PBS in a different storage buffer formulation.

• 

  FC experiment of A431 using 83407-7-RR (same clone as 83407-7-PBS)

  1x10^6 A431 cells were intracellularly stained with 0.25 ug Fez2 Recombinant Antibody (83407-7-RR, Clone:240248B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83407-7-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83407-7-RR

  Biolayer interferometry (BLl) kinetic assays of 83407-7-RR against Human FEZ2 were performed. The affinity constant is below 1 nM.

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Product Information

The immunogen of 83407-7-PBS is FEZ2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FEZ2 fusion protein Ag15370
• Full Name: fasciculation and elongation protein zeta 2 (zygin II)
• Calculated Molecular Weight: 353 aa, 40 kDa
• Observed Molecular Weight: ~50 kDa
• GenBank Accession Number: BC110386
• Gene Symbol: FEZ2
• Gene ID (NCBI): 9637
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FEZ2 is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles.