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FEZ2 Recombinant antibody

FEZ2 Uni-rAbTM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240248B10

Cat no : 83407-7-RR

Synonyms

Zygin-2, Zygin II, Zygin 2, HUM3CL, Fasciculation and elongation protein zeta-2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FEZ2 Recombinant antibody (83407-7-RR)

    WB analysis using 83407-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83407-7-RR (FEZ2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FEZ2 Recombinant antibody (83407-7-RR)

    IHC staining of human ovary cancer using 83407-7-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83407-7-RR (FEZ2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A431 cells using FEZ2 Recombinant antibody (83407-7-RR)

    FC experiment of A431 using 83407-7-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug Fez2 Recombinant Antibody (83407-7-RR, Clone:240248B10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83407-7-RR

    Affinity and Kinetic Characterization of 83407-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83407-7-RR against Human FEZ2 were performed. The affinity constant is below 1 nM.

Tested Applications

Positive WB detected inHeLa cells, Jurkat cells, HepG2 cells, A375 cells
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 83407-7-RR is FEZ2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FEZ2 fusion protein Ag15370
Full Name fasciculation and elongation protein zeta 2 (zygin II)
Calculated Molecular Weight 353 aa, 40 kDa
Observed Molecular Weight~50 kDa
GenBank Accession NumberBC110386
Gene Symbol FEZ2
Gene ID (NCBI) 9637
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FEZ2 is an ortholog of the C. elegans unc-76 gene, which is necessary for normal axonal bundling and elongation within axon bundles.

Protocols

Product Specific Protocols
WB protocol for FEZ2 antibody 83407-7-RRDownload protocol
IHC protocol for FEZ2 antibody 83407-7-RRDownload protocol
FC protocol for FEZ2 antibody 83407-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols