FER Polyclonal antibody, PBS Only

FER Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 25287-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 25287-1-AP (same clone as 25287-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 25287-1-AP (FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A431 using 25287-1-AP (same clone as 25287-1-PBS)

  A431 cells were subjected to SDS PAGE followed by western blot with 25287-1-AP (FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 25287-1-AP (same clone as 25287-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 25287-1-AP (FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 25287-1-AP (same clone as 25287-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 25287-1-AP (FER Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

• 

  IP experiment of MCF-7 using 25287-1-AP (same clone as 25287-1-PBS)

  IP result of anti-FER (IP:25287-1-AP, 4ug; Detection:25287-1-AP 1:1000) with MCF-7 cells lysate 2400ug. This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 25287-1-AP (same clone as 25287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25287-1-AP (FER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 25287-1-AP (same clone as 25287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25287-1-AP (FER antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 25287-1-AP (same clone as 25287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25287-1-AP (FER Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 25287-1-AP (same clone as 25287-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25287-1-AP (FER Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 25287-1-AP (same clone as 25287-1-PBS)

  Immunofluorescent analysis of MCF-7 cells using 25287-1-AP (FER antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

• 

  IF Staining of MCF-7 using 25287-1-AP (same clone as 25287-1-PBS)

  Immunofluorescent analysis of MCF-7 cells using 25287-1-AP (FER antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25287-1-PBS in a different storage buffer formulation.

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Product Information

25287-1-PBS targets FER in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FER fusion protein Ag18173
• Full Name: fer (fps/fes related) tyrosine kinase
• Calculated Molecular Weight: 822 aa, 95 kDa
• Observed Molecular Weight: 95 kDa
• GenBank Accession Number: BC017060
• Gene Symbol: FER
• Gene ID (NCBI): 2241
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

FER(Tyrosine-protein kinase Fer) is also named as TYK3 and belongs to the protein kinase superfamily. Tyrosine-protein kinase acts downstream of cell surface receptors for growth factors and plays a role in the regulation of the actin cytoskeleton, microtubule assembly, lamellipodia formation, cell adhesion, cell migration and chemotaxis. This protein has 3 isoforms produced by alternative promoter usage and alternative splicing.