|Positive WB detected in||HeLa cells, NIH/3T3 cells|
|Positive IP detected in||NIH/3T3 cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||NIH/3T3 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
14768-1-AP targets FEN1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FEN1 fusion protein Ag6552|
|Full Name||flap structure-specific endonuclease 1|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||48 kDa|
|GenBank accession number||BC000323|
|Gene ID (NCBI)||2237|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FEN1(Flap endonuclease-1) is the prototypical member of the 5'-nuclease superfamily, whose activities span a range of cellular pathways involved in DNA replication and genome maintenance (PMID: 22118811, 21496641, 20929870). FEN1 is a structure-selective metallonuclease essential for Okazaki fragment maturation through efficient removal of 5' flaps resulting from strand displacement during lagging-strand synthesis (PMID: 8144677, 9081985). FEN1 is overexpressed in multiple cancer types, and has been suggested both as a biomarker relating to prognosis and disease progression and as a potential therapeutic target (PMID: 19010819, 16879693, 19596913, 27526030).
|Product Specific Protocols|
|WB protocol for FEN1 antibody 14768-1-AP||Download protocol|
|IHC protocol for FEN1 antibody 14768-1-AP||Download protocol|
|IF protocol for FEN1 antibody 14768-1-AP||Download protocol|
|IP protocol for FEN1 antibody 14768-1-AP||Download protocol|
|Click here to view our Standard Protocols|
DNA Repair (Amst)
HIV-1 exploits the Fanconi anemia pathway for viral DNA integration.
Upregulation of FEN1 Is Associated with the Tumor Progression and Prognosis of Hepatocellular Carcinoma.
Am J Physiol Cell Physiol
Succinylation at a key residue of FEN1 is involved in the DNA damage response to maintain genome stability.
In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.
J Mol Cell Biol
SUMO-1 modification of FEN1 facilitates its interaction with Rad9-Rad1-Hus1 to counteract DNA replication stress.