FECH Polyclonal antibody

FECH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

23

Reviews (1)

Cat no : 14466-1-AP

Synonyms

EPP, FCE, FECH, Ferrochelatase, mitochondrial, Heme synthase, Protoheme ferro lyase

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Validation Data Gallery

• 

  WB analysis using 14466-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 14466-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 14466-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 14466-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 14466-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14466-1-AP (FECH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 14466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14466-1-AP (FECH Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 14466-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 14466-1-AP (FECH Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, human liver tissue, HepG2 cells, human heart tissue, K-562 cells, mouse kidney tissue
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 22 publications below
• IHC: See 2 publications below

Product Information

14466-1-AP targets FECH in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FECH fusion protein Ag5814
• Full Name: ferrochelatase (protoporphyria)
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 40-48 kDa
• GenBank Accession Number: BC039841
• Gene Symbol: FECH
• Gene ID (NCBI): 2235
• RRID: AB_2231579
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FECH(ferrochelatase, mitochondrial ) is also named as heme synthase, protoheme ferro-lyase and belongs to the ferrochelatase family. It is a homodimeric (86 kDa) mitochondrial membrane-associated enzyme that catalyzes the insertion of ferrous iron into protoporphyrin to form heme(PMID:11175906). It has 2 isoforms produced by alternative splicing and the full length protein has a transit peptide with 54 amino acids.

Publications

Species	Application	Title
human	WB	Nature; SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells.; Authors - Ying Wang
human	WB	Cancer Discov; NCOA4-Mediated Ferritinophagy Is a Pancreatic Cancer Dependency via Maintenance of Iron Bioavailability for Iron-Sulfur Cluster Proteins; Authors - Naiara Santana-Codina
human	WB	Cell Metab; A Single Adaptable Cochaperone-Scaffold Complex Delivers Nascent Iron-Sulfur Clusters to Mammalian Respiratory Chain Complexes I-III.; Authors - Nunziata Maio
human	WB	Mol Cell; Maintaining Iron Homeostasis Is the Key Role of Lysosomal Acidity for Cell Proliferation.; Authors - Ross A Weber
human	WB	J Am Coll Cardiol; Heme levels are increased in human failing hearts.; Authors - Khechaduri Arineh A
mouse	WB	Nat Commun; PMID: 32019928; Authors - Erika M Palmieri

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marina (Verified Customer) (07-24-2024)

Incubation with primary antibody ON at 4C. Incubation with secondary antibody (rabbit) for 2h at RT.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human cell lines