FDXR Polyclonal antibody

• Featured Product
• KD/KO Validated

FDXR Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 15584-1-AP

Synonyms

Adrenodoxin reductase, ADXR, Aerredoxin Reductase, AR, FDXR, Ferredoxin NADP(+) reductase

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Validation Data Gallery

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  WB analysis using 15584-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15584-1-AP (FDXR antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig adrenal gland using 15584-1-AP

  pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 15584-1-AP (FDXR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 15584-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 15584-1-AP (FDXR antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 15584-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15584-1-AP (FDXR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15584-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15584-1-AP (FDXR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse testis tissue, pig adrenal gland tissue, human adrenal gland tissue, rat testis tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

15584-1-AP targets FDXR in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FDXR fusion protein Ag7145
• Full Name: ferredoxin reductase
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 48-58 kDa
• GenBank Accession Number: BC063493
• Gene Symbol: FDXR
• Gene ID (NCBI): 2232
• RRID: AB_2102602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ferredoxin reductase (FDXR, also known as adrenodoxin reductase) is a mitochondrial membrane-associated flavoprotein. One of its functions is to transfer electrons from NADPH to the two human ferredoxin proteins, FDX1 and FDX2. FDXR is expressed in all tissues with the highest expression in tissues such as the adrenal cortex, that specialize in steroid hormone synthesis. Functionally, FDXR is suggested to be involved in various Functionally, FDXR is suggested to be involved in various. Notably, recent studies have shown that FDXR mutations are associated with mitochondrial disorders, probably due to its role in iron-sulfur cluster protein biosynthesis (PMID: 32304229). FDXR has 7 isoforms with the molecular weight of 48-58 kDa.

Publications

Species	Application	Title
human	WB	J Biol Chem; The neurosteroid pregnenolone is synthesized by a mitochondrial P450 enzyme other than CYP11A1 in human glial cells.; Authors - Yiqi Christina Lin
human	WB	Front Oncol; FDXR drives primary and endocrine-resistant tumor cell growth in ER+ breast cancer via CPT1A-mediated fatty acid oxidation; Authors - Chaojun Yan ; KD Validated