FceR1a Polyclonal antibody

• 

  WB analysis using 10980-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10980-1-AP (Fc Epsilon RI Alpha antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 10980-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 10980-1-AP (Fc epsilon RI alpha antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 10980-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 10980-1-AP (Fc epsilon RI alpha antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

FceR1a Polyclonal Antibody for WB, ELISA

Cat No. 10980-1-AP

Publications(7)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

IgE Fc receptor subunit alpha, FceRIa, FcERI, Fc-epsilon RI-alpha, FCE1A

---

Tested Applications

• Positive WB detected in: MC/9 cells, human spleen tissue, RAW 264.7 cells, mouse spleen tissue, rat spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Product Information

10980-1-AP targets FceR1a in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FceR1a fusion protein Ag1398
• Full Name: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 55-70 kDa
• GenBank Accession Number: BC015195
• Gene Symbol: FceR1a
• Gene ID (NCBI): 2205
• RRID: AB_2103033
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P12319
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316). The calculated molecular weight of FceR1a is 30 kDa, the 55- to 70-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation (PMID: 11344350; 12671054).

Publications

Species	Application	Title
mouse	IF	Science; Response to Comment on "Tumor-initiating cells establish an IL-33-TGF-β niche signaling loop to promote cancer progression".; Authors - Sachiko Taniguchi
human	IHC	J Allergy Clin Immunol; Mast cell-associated alveolar inflammation in patients with atopic uncontrolled asthma.; Authors - Andersson Cecilia K CK
human	WB	J Allergy Clin Immunol; Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces TH2/TH17 polarization through dectin-2.; Authors - Yunsheng Liang
mouse	WB	Transl Res; Allergic asthma aggravates angiotensin Ⅱ-induced cardiac remodeling in mice.; Authors - Chi Geng ; KO Validated
mouse	WB	Theranostics; Elevated IgE promotes cardiac fibrosis by suppressing miR-486a-5p.; Authors - Hongmei Zhao
human	IHC	Thorax; Novel site-specific mast cell subpopulations in the human lung.; Authors - Andersson C K CK