Fc Epsilon RI Alpha Polyclonal antibody
Fc Epsilon RI Alpha Polyclonal Antibody for WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 10980-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | MC/9 cells, human spleen tissue, RAW 264.7 cells, mouse spleen tissue, rat spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 3 publications below |
| IF | See 1 publications below |
Product Information
10980-1-AP targets Fc Epsilon RI Alpha in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Fc Epsilon RI Alpha fusion protein Ag1398 |
| Full Name | Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide |
| Calculated Molecular Weight | 30 kDa |
| Observed Molecular Weight | 55-70 kDa |
| GenBank Accession Number | BC015195 |
| Gene Symbol | Fc Epsilon RI Alpha |
| Gene ID (NCBI) | 2205 |
| RRID | AB_2103033 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fc fragment of IgE, high affinity I, receptor for, alpha polypeptide, also known as high affinity immunoglobulin epsilon receptor subunit alpha, FCER1A and FCE1A, is a single-pass type I membrane protein which contains 2 immunoglobulin-like domains. FCER1A is a subunit of the IgE receptor, which is composed of one glycosylated alpha (FCER1A), one beta (FCER1B), and two gamma (FCER1G) subunits. The high affinity IgE receptor plays a central role in allergic disease, coupling allergen and mast cells to initiate the inflammatory and immediate hypersensitivity responses that are characteristic of disorders such as hay fever and asthma. The calculated molecular weight of FCER1A is 30 kDa, the 55- to 70-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation (PMID: 11344350; 12671054).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Fc Epsilon RI Alpha antibody 10980-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science Response to Comment on "Tumor-initiating cells establish an IL-33-TGF-β niche signaling loop to promote cancer progression". | ||
J Allergy Clin Immunol Mast cell-associated alveolar inflammation in patients with atopic uncontrolled asthma. | ||
J Allergy Clin Immunol Thymic stromal lymphopoietin epigenetically upregulates Fc receptor γ subunit-related receptors on antigen-presenting cells and induces TH2/TH17 polarization through dectin-2. | ||
Transl Res Allergic asthma aggravates angiotensin Ⅱ-induced cardiac remodeling in mice.
| ||
