FCAR Recombinant antibody, PBS Only (Capture)

FCAR Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

241426D1

Cat no : 84384-3-PBS

Synonyms

CD89, IgA Fc receptor, 241426D1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP01257-2

  Sandwich ELISA standard curve of MP01257-2, Human CD89 Recombinant Matched Antibody Pair - PBS only. 84384-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02338. 84384-1-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL

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  Sample test of MP01257-2

  U-937 cells were cultured unstimulated or stimulated with 10 μg/mL PHA and 1 μg/ml ionomycin for 36 hours. The mean CD89 concentration was determined to be 25.5 pg/mL in unstimulated U-937 supernatant, 1,931.0 pg/mL in stimulated U-937 supernatant.

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  Sample test of MP01257-2

  Serum of seven individual healthy human donors was measured. The human CD89 concentration of detected samples was determined to be 319.2 pg/mL with a range of 27.5 - 1,544.1 pg/mL.

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  Cytometric bead array standard curve of MP01257-1

  Cytometric bead array standard curve of MP01257-1, CD89 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84384-3-PBS. Detection antibody: 84384-2-PBS. Standard: RP02338. Range: 0.156-10 ng/mL.

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  Cytometric bead array standard curve of MP01257-2

  Cytometric bead array standard curve of MP01257-2, CD89 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84384-3-PBS. Detection antibody: 84384-1-PBS. Standard: RP02338. Range: 0.156-10 ng/mL.

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Product Information

84384-3-PBS targets FCAR as part of a matched antibody pair:

MP01257-1: 84384-3-PBS capture and 84384-2-PBS detection (validated in Cytometric bead array)

MP01257-2: 84384-3-PBS capture and 84384-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant Protein
• Full Name: Fc fragment of IgA, receptor for
• Calculated Molecular Weight: 287 aa, 32 kDa
• GenBank Accession Number: BC027953
• Gene Symbol: CD89
• Gene ID (NCBI): 2204
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.