FBXW5 Polyclonal antibody, PBS Only

FBXW5 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18321-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 18321-1-AP (same clone as 18321-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18321-1-AP (FBXW5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 18321-1-AP (same clone as 18321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse testis using 18321-1-AP (same clone as 18321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 18321-1-AP (same clone as 18321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 18321-1-AP (same clone as 18321-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18321-1-AP (FBXW5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18321-1-PBS in a different storage buffer formulation.

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Product Information

18321-1-PBS targets FBXW5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXW5 fusion protein Ag13163
• Full Name: F-box and WD repeat domain containing 5
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC014297
• Gene Symbol: FBXW5
• Gene ID (NCBI): 54461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

F-box proteins have been shown to be critical for the ubiquitin-mediated degradation of cellular regulatory proteins, and they are a family of eukaryotic proteins characterized by an approximately 40 amino acid motif. SCF complex, a class of ubiquitin ligases, consists of invariable components, Skp1 and Cullin, and variable components of F-box proteins, which have a primary role in determining substrate specificity. FBXW5 (F-box/WD repeat-containing protein 5) is a novel protein containing WD-40 repeats, which were frequently involved in protein-protein interactions. FBXW5 negatively regulates TAK1 in the IL-1beta signaling pathway. By forming different E3 ubiquitin-protein ligase complexes, FBXW5 mediates the polyubiquitination and subsequent degradation of TSC2 and centriolar protein HsSAS-6.