FBXW12 Polyclonal antibody

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  WB analysis of K-562 using 21687-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21687-1-AP (FBXW12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 21687-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21687-1-AP (FBXW12 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21687-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21687-1-AP (FBXW12 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FBXW12 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21687-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

F box only protein 35, Fbw12, FBXO12, FBXO35, FBXW12

Formulation:  PBS and Azide

PBS and Azide

Conjugate:  Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: K-562 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21687-1-AP targets FBXW12 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FBXW12 fusion protein Ag16266
• Full Name: F-box and WD repeat domain containing 12
• Calculated Molecular Weight: 464 aa, 53 kDa
• Observed Molecular Weight: 53 kDa
• GenBank Accession Number: BC101304
• Gene Symbol: FBXW12
• Gene ID (NCBI): 285231
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6X9E4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.