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FBXW12 Polyclonal antibody

FBXW12 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21687-1-AP

Synonyms

F box only protein 35, Fbw12, FBXO12, FBXO35, FBXW12

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using FBXW12 Polyclonal antibody (21687-1-AP)

    WB analysis of K-562 using 21687-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 21687-1-AP (FBXW12 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FBXW12 Polyclonal antibody (21687-1-AP)

    IHC staining of human breast cancer using 21687-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21687-1-AP (FBXW12 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FBXW12 Polyclonal antibody (21687-1-AP)

    IHC staining of human breast cancer using 21687-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21687-1-AP (FBXW12 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21687-1-AP is FBXW12 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXW12 fusion protein Ag16266
Full Name F-box and WD repeat domain containing 12
Calculated Molecular Weight 464 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC101304
Gene Symbol FBXW12
Gene ID (NCBI) 285231
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.